| 质粒名称: | pBabe puro IRES-mCherry |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 128038 |
| 存储实验室: | Oskar Laur |
|
载体骨架基本信息 |
|
| 载体名称: | pBABE puro |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | - |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBabe puro IRES-mCherry was a gift from Oskar Laur (Addgene plasmid # 128038 ; http://n2t.net/addgene:128038 ; RRID:Addgene_128038) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBabe puro IRES-mCherry 6384 bp ds-DNA circular SYN 27-AUG-2019
DEFINITION Retroviral vector with IRES-mCherry.
ACCESSION .
VERSION .
KEYWORDS pBabe puro IRES-mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6384)
TITLE Emory Custom Cloning Core Plasmids - Oskar Laur
REFERENCE 2 (bases 1 to 6384)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 27, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6384
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 8..477
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 542..741
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 942..1358
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1288..1310
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1326..1342
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature 1405..1991
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(1572..1589)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 1799..1818
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 1992..2702
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(2137..2155)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 2405..2424
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind complement(2714..2734)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2719..3048
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2899..3034
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2961..2980
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3058..3657
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3058..3077)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 3554..3574
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 3878..4347
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(4477..4494)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4648..5236)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4728..4747)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5396..6256)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6019..6038
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6257..6361)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccac gctgcgtctg
1381 tttaaacgcc acgagcatga attccccctc tccctccccc ccccctaacg ttactggccg
1441 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
1501 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
1561 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
1621 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
1681 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
1741 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
1801 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
1861 gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa
1921 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
1981 tggccacaac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
2041 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
2101 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
2161 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
2221 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
2281 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
2341 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
2401 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
2461 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
2521 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
2581 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
2641 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
2701 aggtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
2761 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
2821 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
2881 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
2941 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
3001 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttaccatg
3061 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
3121 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
3181 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
3241 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
3301 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
3361 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
3421 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
3481 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
3541 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
3601 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
3661 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgataaaa
3721 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
3781 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata
3841 gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata
3901 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
3961 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
4021 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
4081 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
4141 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
4201 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
4261 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
4321 tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt
4381 tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg
4441 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
4501 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
4561 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
4621 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
4681 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
4741 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
4801 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
4861 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
4921 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
4981 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
5041 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
5101 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
5161 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
5221 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
5281 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
5341 ccttttgcgg ccgcaaatca atctaaagta tatatgagta aacttggtct gacagttacc
5401 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
5461 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
5521 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
5581 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
5641 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
5701 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
5761 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
5821 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
5881 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
5941 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
6001 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
6061 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
6121 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
6181 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
6241 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
6301 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
6361 gcacatttcc ccgaaaagtg ccac
//
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